https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 DNA damage and repair in the female germline: contributions to ART https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37385 Wed 28 Oct 2020 18:12:02 AEDT ]]> Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19948 Wed 27 Jul 2022 13:58:56 AEST ]]> Mechanistic insight into the regulation of lipoxygenase-driven lipid peroxidation events in human spermatozoa and their impact on male fertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45204 Wed 26 Oct 2022 15:29:09 AEDT ]]> Prospects for immunocontraception in feral horse population control: exploring novel targets for an equine fertility vaccine https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23881 Wed 23 Feb 2022 16:04:29 AEDT ]]> Lipid peroxidation in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27670 Wed 17 May 2017 14:00:30 AEST ]]> Should we be measuring DNA damage in human spermatozoa? New light on an old question https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39598 Wed 15 Jun 2022 12:54:07 AEST ]]> From peptide masses to pregnancy maintenance: a comprehensive proteomic analysis of the early equine embryo secretome, blastocoel fluid, and capsule https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30545 Wed 15 Dec 2021 16:09:36 AEDT ]]> The sins of our forefathers: paternal impacts on De Novo mutation rate and development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47168 Wed 14 Dec 2022 15:48:43 AEDT ]]> RAB-Like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15139 Wed 14 Aug 2024 09:59:14 AEST ]]> Accumulation of electrophilic aldehydes during postovulatory aging of mouse oocytes causes reduced fertility, oxidative stress, and apoptosis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24909 Wed 12 Aug 2020 09:08:42 AEST ]]> More than a simple lock and key mechanism: unraveling the intricacies of sperm-oocyte interactions https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15653 Wed 11 Apr 2018 17:40:40 AEST ]]> Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:24938 Wed 11 Apr 2018 16:25:07 AEST ]]> The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11890 Wed 11 Apr 2018 15:29:17 AEST ]]> Apoptosis and DNA damage in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23215 Wed 11 Apr 2018 15:05:01 AEST ]]> The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27762 in vitro fertilization (IVF) success. Furthermore, reduced expression of HSPA2 from the human sperm proteome leads to an impaired capacity for cumulus matrix dispersal, sperm-egg recognition and fertilization following both IVF and ICSI. In this review, we consider the evidence supporting the role of HSPA2 in sperm function and explore the potential mechanisms by which it is depleted in the spermatozoa of infertile patients. Such information offers novel insights into the molecular mechanisms governing sperm function.]]> Wed 11 Apr 2018 13:54:40 AEST ]]> Rosiglitazone improves stallion sperm motility, ATP content, and mitochondrial function https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30037 Wed 11 Apr 2018 13:40:38 AEST ]]> CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified Ccdc63 as essential for spermiogenesis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26157 Wed 11 Apr 2018 13:31:01 AEST ]]> Electrophilic aldehyde products of lipid peroxidation selectively adduct to heat shock protein 90 and arylsulfatase A in stallion spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:34663 Wed 10 Apr 2019 13:35:43 AEST ]]> Transgenerational inheritance: how impacts to the epigenetic and genetic information of parents affect offspring health https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38138 Wed 04 Aug 2021 15:42:40 AEST ]]> A Comparison Between the Felix™ Electrophoretic System of Sperm Isolation and Conventional Density Gradient Centrifugation: a Multicentre Analysis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50495 Wed 01 May 2024 14:44:13 AEST ]]> Investigation into the presence and functional significance of proinsulin C-peptide in the female germline https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47613 Tue 24 Jan 2023 11:43:18 AEDT ]]> Mass Spectrometry proteomics and the study of sperm cell biology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18188 Tue 23 Jun 2015 18:36:40 AEST ]]> Male reproductive ageing: a radical road to ruin https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54323 Tue 20 Feb 2024 15:59:02 AEDT ]]> The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15122 Tue 13 Aug 2024 10:06:20 AEST ]]> Non-surgical sterilisation methods may offer a sustainable solution to feral horse (Equus caballus) overpopulation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33177 Tue 11 Sep 2018 12:15:39 AEST ]]> Probing the origins of 1,800 MHz radio frequency electromagnetic radiation induced damage in mouse immortalized germ cells and spermatozoa in vitro https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35042 Tue 11 Jun 2019 14:20:01 AEST ]]> Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39106 Tue 10 May 2022 09:39:30 AEST ]]> Impacts of obesity, maternal obesity and nicotinamide mononucleotide supplementation on sperm quality in mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46888 Tue 06 Dec 2022 11:13:27 AEDT ]]> Electromagnetic radiation and oxidative stress in the male germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23922 Tue 01 Nov 2016 10:15:52 AEDT ]]> Impact of oxidative stress on male and female germ cells: implications for fertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39924 Thu 30 Jun 2022 12:02:31 AEST ]]> Conception and early pregnancy in the mare: lipidomics the unexplored frontier https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51207 Thu 24 Aug 2023 15:00:51 AEST ]]> Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52637 Thu 19 Oct 2023 15:11:13 AEDT ]]> Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43344 Thu 15 Sep 2022 15:19:01 AEST ]]> Genomic integrity in the male germ line: evidence in support of the disposable soma hypothesis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38137 cis-platinum (BEP) and (ii) the ageing process. A 3-week exposure to BEP induced complete azoospermia associated with a loss of developing germ cells and extensive vacuolization of Sertoli cell cytoplasm. Following cessation of treatment, spermatozoa first appeared in the caput epididymis after 6 weeks and by 12 weeks motile spermatozoa could be recovered from the cauda, although the count (P < 0.001) and motility (P < 0.01) of these cells were significantly reduced and superoxide generation was significantly elevated (P < 0.001). Despite this increase in free radical generation, no evidence of chromatin instability was detected in these spermatozoa. Furthermore, embryos obtained from females mated at this 12-week time point showed no evidence of an increased mutational load. Similarly, progressive ageing of Big Blue mice had no impact on the quality of the spermatozoa, fertility or mutation frequency in the offspring despite a significant increase in the mutational load carried by somatic tissues such as the liver (P < 0.05). We conclude that the male germ line is highly resistant to mutation in keeping with the disposable soma hypothesis, which posits that genetic integrity in the germ cells will be maintained at the expense of the soma, in light of the former’s sentinel position in safeguarding the stability of the genome.]]> Thu 12 Aug 2021 15:36:47 AEST ]]> Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49319 Thu 11 May 2023 14:53:05 AEST ]]> Swim-up of tammar wallaby (Macropus eugenii) spermatozoa in Biggers, Whitter and Whittingham (BWW) medium: maximisation of sperm motility, minimisation of impairment of sperm metabolism and induction of sperm hyperactivation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33765 Thu 10 Jan 2019 17:54:36 AEDT ]]> Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32118 Thu 03 May 2018 12:11:55 AEST ]]> Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced protein damage in male germ cells https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32117 Thu 03 May 2018 12:04:54 AEST ]]> Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42690 Thu 01 Sep 2022 09:06:31 AEST ]]> The chaperonin containing TCP1 complex (CCT/TRiC) involved in mediating sperm-oocyte interaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11888 Sat 24 Mar 2018 10:31:26 AEDT ]]> Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9572 Sat 24 Mar 2018 08:34:47 AEDT ]]> Proteomic and functional analysis of human sperm detergent resistant membranes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:13040 Sat 24 Mar 2018 08:16:38 AEDT ]]> Label-free quantitation of phosphopeptide changes during rat sperm capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:9963 Sat 24 Mar 2018 08:14:26 AEDT ]]> Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10935 Sat 24 Mar 2018 08:13:21 AEDT ]]> Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11205 Sat 24 Mar 2018 08:12:44 AEDT ]]> New insights into sperm physiology and pathology https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11318 Sat 24 Mar 2018 08:12:33 AEDT ]]> Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10106 Sat 24 Mar 2018 08:07:16 AEDT ]]> Paternal obesity, interventions, and mechanistic pathways to impaired health in offspring https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20202 Sat 24 Mar 2018 08:06:49 AEDT ]]> The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17174 Sat 24 Mar 2018 08:06:30 AEDT ]]> Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17478 −/−-deficient mice. In the caput epididymis of Ido1−/− animals, the lack of IDO activity was not compensated by other tryptophan-catabolizing enzymes and led to the loss of kynurenine production. The absence of IDO generated an inflammatory state in the caput epididymis as revealed by an increased accumulation of various inflammation markers. The absence of IDO also increased the tryptophan content of the caput epididymis and generated a parallel increase in caput epididymal protein content as a consequence of deficient proteasomal activity. Surprisingly, the lack of IDO expression had no noticeable impact on overall male fertility but did induce highly significant increases in both the number and the percentage of abnormal spermatozoa. These changes coincided with a significant decrease in white blood cell count in epididymal fluid compared with wild type mice. These data provide support for IDO playing a hitherto unsuspected role in sperm quality control in the epididymis involving the ubiquitination of defective spermatozoa and their subsequent removal.]]> Sat 24 Mar 2018 08:04:10 AEDT ]]> Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17528 Sat 24 Mar 2018 08:03:55 AEDT ]]> The John Hughes Memorial Lecture: aspects of sperm physiology-oxidative stress and the functionality of stallion spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20539 Sat 24 Mar 2018 08:02:43 AEDT ]]> Sperm capacitation: a distant landscape glimpsed but unexplored https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17300 Sat 24 Mar 2018 08:01:46 AEDT ]]> The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17419 Sat 24 Mar 2018 08:01:38 AEDT ]]> Capacitation in the presence of methyl-β-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:18685 Sat 24 Mar 2018 08:01:06 AEDT ]]> Melatonin prevents postovulatory oocyte aging in the mouse and extends the window for optimal fertilization in vitro https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20252 Sat 24 Mar 2018 07:59:55 AEDT ]]> Oxidative stress and ageing of the post-ovulatory oocyte https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19945 Sat 24 Mar 2018 07:58:35 AEDT ]]> Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19949 Sat 24 Mar 2018 07:58:32 AEDT ]]> The impact of sperm DNA damage in assisted conception and beyond: recent advances in diagnosis and treatment https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:19950 Sat 24 Mar 2018 07:58:32 AEDT ]]> Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17890 Sat 24 Mar 2018 07:56:25 AEDT ]]> Apoptosis in the germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17960 Sat 24 Mar 2018 07:56:21 AEDT ]]> Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20323 Sat 24 Mar 2018 07:55:12 AEDT ]]> Age, the environment and our reproductive future: bonking baby boomers and the future of sex https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21363 Sat 24 Mar 2018 07:51:26 AEDT ]]> Oxidative stress, spermatozoa and leukocytic infiltration: relationships forged by the opposing forces of microbial invasion and the search for perfection https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20031 Sat 24 Mar 2018 07:50:54 AEDT ]]> Head and flagella subcompartmental proteomic analysis of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20019 Sat 24 Mar 2018 07:50:53 AEDT ]]> Biochemical alterations in the oocyte in support of early embryonic development https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29633 2+2+ release and the suite of molecular modifications that sweep through the oocyte to ensure the successful union of the parental germlines and ensure embryogenic fidelity.]]> Sat 24 Mar 2018 07:41:53 AEDT ]]> Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27766 Sat 24 Mar 2018 07:40:49 AEDT ]]> CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:29442 Sat 24 Mar 2018 07:39:22 AEDT ]]> Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27976 50 = 6.7 nM) led to a significant reduction in dynamin phosphorylation (10.3% vs. 27.3%; P < 0.001), acrosomal exocytosis (9.7% vs. 25.7%; P < 0.01), and in vitro fertilization (53% vs. 100%; P < 0.01). GSK3 was shown to be present in developing germ cells where it colocalized with dynamin in the peri-acrosomal domain. However, additional GSK3 was acquired by maturing mouse spermatozoa within the male reproductive tract, via a novel mechanism involving direct interaction of sperm heads with extracellular structures known as epididymal dense bodies. These data reveal a novel mode for the cellular acquisition of a protein kinase and identify a key role for GSK3 in the regulation of sperm maturation and acrosomal exocytosis.—Reid, A. T., Anderson, A. L., Roman, S. D., McLaughlin, E. A., McCluskey, A., Robinson, P. J., Aitken, R. J., Nixon, B. Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation.]]> Sat 24 Mar 2018 07:38:43 AEDT ]]> Electrophoretic sperm isolation: optimization of electrophoresis conditions and impact on oxidative stress https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25242 Sat 24 Mar 2018 07:38:18 AEDT ]]> Boronate probes for the detection of hydrogen peroxide release from human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27394 Sat 24 Mar 2018 07:34:11 AEDT ]]> Characterization of an L-amino acid oxidase in equine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25432 Sat 24 Mar 2018 07:31:54 AEDT ]]> Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27535 Sat 24 Mar 2018 07:28:58 AEDT ]]> The impact of oxidative stress on chaperone-mediated human sperm-egg interaction https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26416 Sat 24 Mar 2018 07:27:57 AEDT ]]> Direct methods for the detection of reactive oxygen species in human semen samples https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27751 Sat 24 Mar 2018 07:27:46 AEDT ]]> Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26581 Sat 24 Mar 2018 07:26:12 AEDT ]]> Investigation of the stallion sperm proteome by mass spectrometry https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27065 Sat 24 Mar 2018 07:25:20 AEDT ]]> Proteomic characterization of pig sperm anterior head plasma membrane reveals roles of acrosomal proteins in ZP3 binding https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27056 Sat 24 Mar 2018 07:25:20 AEDT ]]> Oxidative DNA damage in mouse sperm chromosomes: size matters https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26315 Sat 24 Mar 2018 07:24:13 AEDT ]]> A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:26537 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.]]> Sat 24 Mar 2018 07:23:27 AEDT ]]> Proteomic insights into the maturation and capacitation of mammalian spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22257 Sat 24 Mar 2018 07:17:39 AEDT ]]> Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22263 in vitro induction of acrosomal exocytosis by progesterone, but not by the calcium ionophore A23187, and elicited a concomitant reduction of in vitro fertilization. In vivo treatment with these inhibitors also resulted in spermatozoa displaying reduced acrosome reaction potential. Dynamin 1 and 2 phosphorylation increased on progesterone treatment, and this was also selectively blocked by dynasore. On the basis of our collective data, we propose that dynamin could regulate specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.]]> Sat 24 Mar 2018 07:17:38 AEDT ]]> Reactive oxygen species and sperm function-in sickness and in health https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22254 Sat 24 Mar 2018 07:17:34 AEDT ]]> Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22207 R²= 0.89) in untreated populations of human spermatozoa emphasized the pathophysiological significance of these findings. The latter also provide a biochemical explanation for the self-perpetuating nature of oxidative stress in the male germ line, with the products of lipid peroxidation stimulating free radical generation by the sperm mitochondria in a positive feedback loop.]]> Sat 24 Mar 2018 07:11:16 AEDT ]]> OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Oxidative stress and in vitro ageing of the post-ovulatory oocyte: an update on recent advances in the field https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51259 Mon 28 Aug 2023 13:50:03 AEST ]]> Storing stallion sperm in SpermSafe™ at 17°C may improve fertility by reducing mPTP formation https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51973 Mon 25 Sep 2023 10:52:32 AEST ]]> Double strand break DNA repair occurs via non-homologous end-joining in mouse MII oocytes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32871 in vitro fertilisation, though displayed reduced developmental competence beyond the 2-cell stage (P=0.02). To account for these findings, we analysed the efficacy of DSB resolution, revealing a significant reduction in DSB lesions 4 h post-ETP treatment. Notably, this response was completely abrogated by pharmacological inhibition of key elements (DNA-PKcs and DNA ligase IV) of the canonical non-homologous end joining DNA repair pathway, thus providing the first evidence implicating this reparative cascade in the protection of the maternal genome.]]> Mon 23 Sep 2019 10:15:15 AEST ]]> Heat exposure induces oxidative stress and DNA damage in the male germ line https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47477 0.05). Collectively, our acute heat stress model supports the existence of heat susceptible stages of germ cell development, with the round spermatids being most perturbed and spermatogonial stem cells exhibiting resistance to this insult. Such findings were complemented by our chronic heat stress model, which further supported the vulnerability of the round spermatid population.]]> Mon 23 Jan 2023 11:33:24 AEDT ]]> CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46955 Mon 12 Dec 2022 09:05:31 AEDT ]]> Evidence that extrapancreatic insulin production is involved in the mediation of sperm survival https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40400 Mon 11 Jul 2022 13:55:46 AEST ]]> The changing tide of human fertility https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46738 Mon 10 Jul 2023 13:53:20 AEST ]]> Proteomic analysis of human spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:33479 Mon 05 Nov 2018 11:10:42 AEDT ]]> GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36447 Mon 04 May 2020 16:14:04 AEST ]]> Male infertility and somatic health — insights into lipid damage as a mechanistic link https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51402 Mon 01 Jul 2024 14:52:12 AEST ]]> Role of Oxidative Stress in the Etiology of Male Infertility and the Potential Therapeutic Value of Antioxidants https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43788 Fri 30 Sep 2022 08:32:34 AEST ]]> The impact of oxidative stress on reproduction: a focus on gametogenesis and fertilization https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49666 Fri 26 May 2023 11:37:51 AEST ]]> Current global status of male reproductive health https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:55389 Fri 24 May 2024 10:34:53 AEST ]]> Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36974 Fri 24 Jul 2020 14:55:41 AEST ]]> Blockade of ROS production inhibits oncogenic signaling in acute myeloid leukemia and amplifies response to precision therapies https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51529 Fri 08 Sep 2023 12:10:33 AEST ]]>